Review



ethidium homodimer-ii e3599  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier
Bioz Manufacturer Symbol Thermo Fisher manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 90
      Buy from Supplier

    Structured Review

    Thermo Fisher ethidium homodimer-ii e3599
    Ethidium Homodimer Ii E3599, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ethidium+homodimer+ii/ethd+2++e3599/pm34796169-86-17-24
    Average 90 stars, based on 1 article reviews
    ethidium homodimer-ii e3599 - by Bioz Stars, 2026-09
    90/100 stars

    Images

    Related Articles

    Incubation:

    Article Title: Global cell-by-cell evaluation of endothelial viability after two methods of graft preparation in Descemet membrane endothelial keratoplasty
    Article Snippet: .. Each sample was covered with 250 μL of BSS containing Hoechst 33342 (10 μM), ethidium homodimer II(4 μM) and calcein-AM (2 μM) (Invitrogen, Carlsbad, California, USA) and incubated at 37°C for 30 min. Hoechst 33342 and ethidium homodimer II are nuclear binding dyes that do not fluoresce until they are bound to nuclear DNA. ..

    Binding Assay:

    Article Title: Global cell-by-cell evaluation of endothelial viability after two methods of graft preparation in Descemet membrane endothelial keratoplasty
    Article Snippet: .. Each sample was covered with 250 μL of BSS containing Hoechst 33342 (10 μM), ethidium homodimer II(4 μM) and calcein-AM (2 μM) (Invitrogen, Carlsbad, California, USA) and incubated at 37°C for 30 min. Hoechst 33342 and ethidium homodimer II are nuclear binding dyes that do not fluoresce until they are bound to nuclear DNA. ..

    Injection:

    Article Title: Optic Nerve Crush: Axonal Responses in Wild-Type and bcl-2 Transgenic Mice
    Article Snippet: .. The left retinas of three wt and three bcl-2 animals that had received crush of the left optic nerves 4 weeks before, plus the retinas of three wt that had received an injection of IN-1 hybridoma cells, were stained as whole mounts with 2 mM ethidium homodimer II (Molecular Probes) and examined at the confocal microscope. ..

    Staining:

    Article Title: Optic Nerve Crush: Axonal Responses in Wild-Type and bcl-2 Transgenic Mice
    Article Snippet: .. The left retinas of three wt and three bcl-2 animals that had received crush of the left optic nerves 4 weeks before, plus the retinas of three wt that had received an injection of IN-1 hybridoma cells, were stained as whole mounts with 2 mM ethidium homodimer II (Molecular Probes) and examined at the confocal microscope. ..

    Microscopy:

    Article Title: Optic Nerve Crush: Axonal Responses in Wild-Type and bcl-2 Transgenic Mice
    Article Snippet: .. The left retinas of three wt and three bcl-2 animals that had received crush of the left optic nerves 4 weeks before, plus the retinas of three wt that had received an injection of IN-1 hybridoma cells, were stained as whole mounts with 2 mM ethidium homodimer II (Molecular Probes) and examined at the confocal microscope. ..



    Similar Products

    ethidium homodimer ii  (Biotium)
    95
    Biotium ethidium homodimer ii
    Ethidium Homodimer Ii, supplied by Biotium, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ethidium+homodimer+ii/Ethidium+Homodimer+I/pm41645718-517-20-23
    Average 95 stars, based on 1 article reviews
    ethidium homodimer ii - by Bioz Stars, 2026-09
    95/100 stars
    		  Buy from Supplier
    ethidium homodimer-ii e3599  (Thermo Fisher)
    90
    Thermo Fisher ethidium homodimer-ii e3599
    Ethidium Homodimer Ii E3599, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ethidium+homodimer+ii/ethd+2++e3599/pm34796169-86-17-24
    Average 90 stars, based on 1 article reviews
    ethidium homodimer-ii e3599 - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    ethidium homodimer ii  (Thermo Fisher)
    86
    Thermo Fisher ethidium homodimer ii
    (A) <t>TRITC/ethidium</t> channel image showing manual demarcation of graft edge using the polygon selection toll in Image J (yellow outline). (B) Yellow line defined in the TRITC/ethidium image is copied and applied to the FITC/calcein channel image. The edge of calcein-positive area is defined in the same way as the graft edge (blue outline). The difference in the areas of the yellow and blue selections was defined as the trephination damage area. (C) The FITC/calcein channel image is thresholded and segmented. The area of living cells (red area) is used to create a selection mask (green lines). (D) Ultraviolet/Hoechst channel images were colour inverted and the viable graft area selection applied (green outline). Each nucleus (black dot) within the selection mask is counted. Zoomed in area (top right) showing all nuclei within the mask have been counted, as shown by the presence of a red dot in the centre of nucleus. Nuclei outside the mask are not counted (no red dot in centre of nucleus). (E) TRITC/ethidium channel image is inverted, and the living area selection is applied (green outline). Each nucleus (black dot) within the selection mask is counted. Zoomed in area (top right) showing ethidium-staining nuclei within the mask have been counted, as shown by the presence of a red dot in the centre of nucleus.
    Ethidium Homodimer Ii, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ethidium+homodimer+ii/pmc04819631-76-14-22
    Average 86 stars, based on 1 article reviews
    ethidium homodimer ii - by Bioz Stars, 2026-09
    86/100 stars
    		  Buy from Supplier

    Image Search Results


    (A) TRITC/ethidium channel image showing manual demarcation of graft edge using the polygon selection toll in Image J (yellow outline). (B) Yellow line defined in the TRITC/ethidium image is copied and applied to the FITC/calcein channel image. The edge of calcein-positive area is defined in the same way as the graft edge (blue outline). The difference in the areas of the yellow and blue selections was defined as the trephination damage area. (C) The FITC/calcein channel image is thresholded and segmented. The area of living cells (red area) is used to create a selection mask (green lines). (D) Ultraviolet/Hoechst channel images were colour inverted and the viable graft area selection applied (green outline). Each nucleus (black dot) within the selection mask is counted. Zoomed in area (top right) showing all nuclei within the mask have been counted, as shown by the presence of a red dot in the centre of nucleus. Nuclei outside the mask are not counted (no red dot in centre of nucleus). (E) TRITC/ethidium channel image is inverted, and the living area selection is applied (green outline). Each nucleus (black dot) within the selection mask is counted. Zoomed in area (top right) showing ethidium-staining nuclei within the mask have been counted, as shown by the presence of a red dot in the centre of nucleus.

    Journal: The British Journal of Ophthalmology

    Article Title: Global cell-by-cell evaluation of endothelial viability after two methods of graft preparation in Descemet membrane endothelial keratoplasty

    doi: 10.1136/bjophthalmol-2015-307534

    Figure Lengend Snippet: (A) TRITC/ethidium channel image showing manual demarcation of graft edge using the polygon selection toll in Image J (yellow outline). (B) Yellow line defined in the TRITC/ethidium image is copied and applied to the FITC/calcein channel image. The edge of calcein-positive area is defined in the same way as the graft edge (blue outline). The difference in the areas of the yellow and blue selections was defined as the trephination damage area. (C) The FITC/calcein channel image is thresholded and segmented. The area of living cells (red area) is used to create a selection mask (green lines). (D) Ultraviolet/Hoechst channel images were colour inverted and the viable graft area selection applied (green outline). Each nucleus (black dot) within the selection mask is counted. Zoomed in area (top right) showing all nuclei within the mask have been counted, as shown by the presence of a red dot in the centre of nucleus. Nuclei outside the mask are not counted (no red dot in centre of nucleus). (E) TRITC/ethidium channel image is inverted, and the living area selection is applied (green outline). Each nucleus (black dot) within the selection mask is counted. Zoomed in area (top right) showing ethidium-staining nuclei within the mask have been counted, as shown by the presence of a red dot in the centre of nucleus.

    Article Snippet: Each sample was covered with 250 μL of BSS containing Hoechst 33342 (10 μM), ethidium homodimer II(4 μM) and calcein-AM (2 μM) (Invitrogen, Carlsbad, California, USA) and incubated at 37°C for 30 min. Hoechst 33342 and ethidium homodimer II are nuclear binding dyes that do not fluoresce until they are bound to nuclear DNA.

    Techniques: Selection, Staining